en پژوهشي Research <strong>Background: </strong><em>Pseudomonas aeruginosa</em> has become the most common cause of<br> &nbsp;infections in burn patients. The aim of this study was to investigate the antibiotyping and &nbsp;genotyping &nbsp;of &nbsp;<a name="OLE_LINK2"></a><a name="OLE_LINK1"><em>P.&nbsp; aeruginosa</em></a> isolated from burn patients, Mottahari hospital between June- October 2016.&nbsp;<br> <strong>Materials and Methods: </strong>A total of 78&nbsp; <em>P.&nbsp; aeruginosa</em> were collected from wound infected patients. Identification of isolates was performed by biochemical tests and &nbsp;confirmed&nbsp; by specific 16srDNA PCR. Antimicrobial susceptibility test was done by disk diffusion method &nbsp;according with CLSI guidelines. The isolates were then evaluated for genotyping by ERIC-PCR.<br> <strong>Results:</strong> A totally, collected 78 isolates, 77 isolates (98.7%) confirmed as <em>P. aeruginosa </em>by specific PCR. We found 4 antibiotypes, The &nbsp;highest resistance&nbsp; was observed&nbsp; to imipenem and gentamicin (~100%) and the&nbsp; most sensitivity were&nbsp; discovered&nbsp; to&nbsp; colistin &nbsp;(100%). Overally, MDR phenotype&nbsp; were observed in most&nbsp; isolates (98.7%). The PCR of ERIC box produced 52 different patterns and 3 main clusters, 59 (83%), 2 (3%) and 9 (13%) isolates included in cluster A, B and C, respectively and cluster A is predominant ERIC profile. &nbsp;<br> <strong>Discussion:</strong> The high resistance to antibiotic in our study may be &nbsp;due&nbsp; to their&nbsp; abundant&nbsp; use&nbsp; as&nbsp; the&nbsp; prophylactic or treatment&nbsp; regimen in wound infections then appropriate use of antibiotics seems necessary and &nbsp;colistin is a proper choice for treatment of burn infection. In genotyping was shown 3 main clusters and 52 different patterns. Then, the majority&nbsp; of&nbsp; <em>P.&nbsp; aeruginosa</em>&nbsp; isolated&nbsp; from&nbsp; burn&nbsp;&nbsp; patients were related and belonged to cluster A. P. aeruginosa, burns and genotyping technique 31 35 http://journalsystem.ir/browse.php?a_code=A-10-31-3&slc_lang=en&sid=1 Hossein Fazeli 1003194753284600176 1003194753284600176 No Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical sciences, Isfahan, Iran Bahram Nasr Esfahani 1003194753284600177 1003194753284600177 No Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical sciences, Isfahan, Iran Mahboubeh Sattarzadeh 1003194753284600178 1003194753284600178 No Motahhari Burn Hospital, Iran University of Medical Sciences, Tehran, Iran Hajar Mohammadi Barzelighi Hajar_mohamadi@yahoo.com 1003194753284600179 1003194753284600179 Yes Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical sciences, Isfahan, Iran