TY - JOUR JF - SABA-JMS JO - Infect Epidemiol Med VL - 2 IS - 2 PY - 2016 Y1 - 2016/4/01 TI - Antibiotyping and Genotyping of Pseudomonas aeruginosa isolated from Mottahari hospital in Tehran, Iran by ERIC-PCR TT - N2 - Background: Pseudomonas aeruginosa has become the most common cause of infections in burn patients. The aim of this study was to investigate the antibiotyping and genotyping of P. aeruginosa isolated from burn patients, Mottahari hospital between June- October 2016. Materials and Methods: A total of 78 P. aeruginosa were collected from wound infected patients. Identification of isolates was performed by biochemical tests and confirmed by specific 16srDNA PCR. Antimicrobial susceptibility test was done by disk diffusion method according with CLSI guidelines. The isolates were then evaluated for genotyping by ERIC-PCR. Results: A totally, collected 78 isolates, 77 isolates (98.7%) confirmed as P. aeruginosa by specific PCR. We found 4 antibiotypes, The highest resistance was observed to imipenem and gentamicin (~100%) and the most sensitivity were discovered to colistin (100%). Overally, MDR phenotype were observed in most isolates (98.7%). The PCR of ERIC box produced 52 different patterns and 3 main clusters, 59 (83%), 2 (3%) and 9 (13%) isolates included in cluster A, B and C, respectively and cluster A is predominant ERIC profile. Discussion: The high resistance to antibiotic in our study may be due to their abundant use as the prophylactic or treatment regimen in wound infections then appropriate use of antibiotics seems necessary and colistin is a proper choice for treatment of burn infection. In genotyping was shown 3 main clusters and 52 different patterns. Then, the majority of P. aeruginosa isolated from burn patients were related and belonged to cluster A. SP - 31 EP - 35 AU - Fazeli, Hossein AU - Nasr Esfahani, Bahram AU - Sattarzadeh, Mahboubeh AU - Mohammadi Barzelighi, Hajar AD - Department of Bacteriology, Faculty of Medicine, Isfahan University of Medical sciences, Isfahan, Iran KW - P. aeruginosa KW - burns and genotyping technique UR - http://journalsystem.ir/article-1-61-en.html DO - 10.7508/iem.2016.01.003 ER -